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Description
human bsep vesicle kit hek293Das Human BSEP Vesicle Kit enthlt Inside out Membranvesikel aus HEK293 Zellen, die transient die Bile Salt Export Pump (BSEP ABCB11) berexprimieren. BSEP ist der primre hepatische Efflux Transporter fr Gallensalze und ein zentrales Zielprotein in der Risikoabschtzung fr medikamenteninduzierte Cholestase (DILI). Das Kit ist konform mit den FDA (2017) und EMA (2013) Leitlinien fr DDI Studien. Hergestellt von Cell4Pharma. Exklusiv im DACH Raum erhltlich
Das Human BSEP Vesicle Kit enthält Inside-out Membranvesikel aus HEK293-Zellen, die transient die Bile Salt Export Pump (BSEP / ABCB11) überexprimieren. BSEP ist der primäre hepatische Efflux-Transporter für Gallensalze und ein zentrales Zielprotein in der Risikoabschätzung für medikamenteninduzierte Cholestase (DILI). Das Kit ist konform mit den FDA (2017)- und EMA (2013) Leitlinien für DDI Studien.
Hergestellt von Cell4Pharma. Exklusiv im DACH-Raum erhältlich über SeamlessBio.
Transporter-Profil
Name: BSEP, Bile Salt Export Pump, ABCB11, ATP-bindende Kassette Unterfamilie B Mitglied 11
Gen: ABCB11
ATP/AMP-Ratio: >10 (garantiert)
Gewebsexpression: Kanalikuläre Plasmamembran der Hepatozyten
Erkrankungen: Erbliche und medikamenteninduzierte Cholestase (DILI)
Bekannte Substrate: Taurocholat, Pravastatin, Taurolithocholyl-3-Sulfat, konjugierte monovalente Gallensalze
In stock: Human, Katze, Hund
Kit-Inhalt (100 Reaktionen)
- Vesikel-Vial (100 Reaktionen), BSEP überexprimierende HEK293-abgeleitete Vesikel
- AMP-ATP-Lösung (100 mM), Cell4Pharma
- Assay-Puffer, Cell4Pharma
Anwendungen
- allensalztransport-Inhibitionsstudien (Cholestase-Risikoabschätzung)
- Drug-Induced Liver Injury (DILI) Screening
- Drug-Drug-Interaction-(DDI)-Studien gemäß FDA/EMA-Leitlinien
- Substratidentifikation für hepatische Efflux-Transporter
- High-Throughput-Screening (Radiolabel-, Fluoreszenz-, LC-MS-Detektion)
Regulatorische Konformität
Die Hemmung von BSEP ist ein etablierter Mechanismus der medikamenteninduzierten cholestatischen Leberschädigung. Die Bewertung der BSEP-Hemmung wird in den FDA (2017)- und EMA (2013)-DDI-Leitlinien als Teil des Transporter-Charakterisierungspakets für neue Molekülentitäten (NMEs) in der klinischen Entwicklung empfohlen.
Technische Spezifikationen
Wirtszelle: HEK293 (humane embryonale Nierenzelle)
Vesikeltyp: Inside-out-Membranvesikel
Format: 100 Reaktionen pro Kit
Detektion: Radiolabel / Fluoreszenz / LC-MS kompatibel
Lagerung: –80 °C
Verwandte Produkte
- Human Control Vesicle Kit (C4PVK009) – empfohlen als Negativkontrolle in BCRP-Transportassays
- Human MRP2 Vesicle Kit (C4PVK002) – for parallel hepatic efflux assessment
- Human BCRP Vesicle Kit (C4PVK006) – for complete efflux transporter panel
- ciPTEC-OAT1 Assay-Ready Cells – für zellbasierte Nephrotoxizitäts- und Transporterstudien
- Human Serum AB Male OTC (SeamlessBio) – zur Supplementierung von ciPTEC-Kulturmedien
Alle Produkte auf Anfrage.
Kontakt: [email protected] | Telefon: +49 851 37932226
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4.1 ★★★★★
Based on 1212 reviews
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Product Reviews
★★★★★ 5
Unlock your machine's potential
***Twist the WDT tool to remove dont try to just pull, or use your finger nails A slight twist will break the magnetic bond and the tool just comes out of the handle***
i got this to get away chemicals, hot acidic coffee possibly leaching plastic and aluminum that the stock portifilter is made from. This seems like a nice stainless by the looks and heft of it. Aluminium is bad for the brain and plastic is just bad.
Function... Im not using a 200$ or more grinder or anything... Just fresh beans and a 30$ wancle amazon burr grinder and this is pulling beautiful bottomless shots that really helped me dial in better and see my channeling issues that intern lets me adjust and get better.
The stock tapered basket prevents consistant tamps and keeps you from using off the shelf filter papers at the bottom of the basket.
i have to use a gram or 2 more for my dose but that just means stronger drinks. I can tell visually im getting more extraction now that im dialed in by the color after adding milk.
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Reviewed in the United States on March 27, 2026
★★★★★ 1
It doesn’t even fit
It doesn’t fit our Breville Barista Pro because the portafilter head is too tall. Such a disappointment.
Update: Absolutely horrible customer support as well. Their only attempt to rectify the situation was by double checking their own portafilter they have and confirming it works. It’s equivalent of “we investigated ourselves and found nothing wrong.” No replacements, no willingness to accept constructive criticism in order to change the dimensions slightly by decreasing the height of the portafilter head by even a millimeter to make it work, nothing. Just a slap in the face telling me to return it and be gone. Avoid this product.
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Reviewed in the United States on January 4, 2025
★★★★★ 4
More work than it’s worth
It was great quality and the weight was nice, but it was odd having the open bottom and no spout to dribble into a cup when making an espresso. It was difficult to get the wire tool out of the end of the handle, making it difficult to use. I was not that impressed and it’s quite expensive.
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Reviewed in the United States on March 5, 2025
★★★★★ 5
Quality and Function
Looks like a silly little thing, I am not a coffee enthusiast. But it does exactly what it is supposed to do and with high quality. The device (a stretch to call it that) is made of sturdy materials including the needles. They give you two different thicknesses of needles, one for expressi (0.3) and one set for coffee grinds (0.4). Each comes wth some spares. The stand is well designed and keeps grinds from spreading all over. It is very elegant design. I use this with a dosing funnel on my portafilter. Not the cheapest one could buy but well worth the money.
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Reviewed in the United States on March 16, 2025
★★★★★ 5
Well made, high quality product.
This product demonstrates excellent quality, featuring a robust construction and intuitive design for ease of use. It includes two distinct wire sizes and offers straightforward assembly. Its superior durability and strong magnetic properties ensure effortless reattachment to its stand.
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Reviewed in the United States on June 30, 2025