3alpha-Androstanediol (3a-Diol G) ELISA Kit

3alpha-Androstanediol (3a-Diol G) ELISA KitThe 3alpha Diol G ELISA kit is a competitive ELISA to measure 3alpha androstanediol glucuronide in serum. Competition occurs between 3alpha Diol G present in calibrators, controls, specimen samples and an enzyme labelled antigen (HRP conjugate) for a limited number of anti 3alpha Diol G antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with

The 3alpha-Diol G ELISA kit is a competitive ELISA to measure 3alpha-androstanediol glucuronide in serum. Competition occurs between 3alpha-Diol G present in calibrators, controls, specimen samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-3alpha-Diol G antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of 3alpha-Diol G present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the color from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of 3alpha-Diol G in specimen samples and controls can be directly read.