SKU: 59377458956

Human WISP3 (WNT1-inducible-signaling pathway protein 3) ELISA Kit

$234.00 $260.00
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Description

Human WISP3 (WNT1-inducible-signaling pathway protein 3) ELISA KitThe Human WISP3 (WNT1 inducible signaling pathway protein 3) ELISA Kit measures WISP3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti WISP3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human WISP3 (WNT1-inducible-signaling pathway protein 3) ELISA Kit measures WISP3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti WISP3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with WISP3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of WISP3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 59377458956

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4.3 ★★★★★
Based on 2481 reviews
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DS
Natrona Heights, US
★★★★★ 4
Good for a few weeks for large dogs
Color: Blue
It held up for a few weeks. The arms and tip of the tail were chewed off. Had to throw it away so my dogs wouldnt eat the parts that they chewed off. It kept them busy for a while.
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Reviewed in the United States on June 12, 2026
B
Verified Purchase
Betty and Bill H.
Lowell, US
★★★★★ 3
Dog likes it, but is very hard material around smaller dogs when he is playing and running
Color: Blue
My 22lb dog loves this chew. We have owned it just for one day. He immediately loved it and he races through the house holding it in his mouth like a grand prize. I have concerns to the hardness of the ends of it, the material is very hard, and the tail comes to an end shape that could hurt other pets in a multidog home if it hit or stuck in them. I have two smaller chihuahua dogs, one who has lost an eye so this is something to be careful about with other dogs running with this toy. This may not affect a one dog home. Best to observe the dog while playing with it around others.
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Reviewed in the United States on May 6, 2026
B
Verified Purchase
barb belshe
Los Angeles, US
★★★★★ 5
Withstands very aggressive chewing
Color: Blue
We have a very active and aggressive chewer. Items identified for aggressive chewers are “Gone in 60 Seconds”. This toy is amazing. It has withstood Jake’s many attempts to destroy it and is one of his favorites! It is not hard like acrylic but is hard rubber with some give decreasing risk of fractured teeth. Best toy yet!!!
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Reviewed in the United States on May 8, 2026
C
Verified Purchase
Candace McCullough
Louisville, US
★★★★★ 5
Great chew toy
Color: Blue
I bought this almost a year go. My dog is still chewing on it! Nothing last this long with her. She is a 100lb Rottweiler that is a strong chewer.
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Reviewed in the United States on June 12, 2026
D
Verified Purchase
Dawn Clegg
Pawtucket, US
★★★★★ 4
Well loved
Color: Blue, Color: Blue
We just got this sweet girl from the shelter a few days ago. She's had this toy for 2 days and already chomped off one of the legs. She does have other toys so she doesn't play with this one all the time but she does like it a lot! It's very hard and durable on the ends. But as you can see, it's not indestructible. She carries this little guy around the house like they're BFFs. It's super adorable.
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Reviewed in the United States on May 21, 2026

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