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Description
Human ATGL ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes and remove the supernatant for analysis. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, but avoid repeated freeze-thaw cycles. Pre-Assay Preparation: 1. Remove the reagent kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 20ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube is used as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with adipose triglyceride lipase (ATGL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of adipose triglyceride lipase (ATGL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human adipose triglyceride lipase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Adipose triglyceride lipase, also known as protease domain-containing protein 2 and ATGL, is an enzyme encoded by the PNPLA2 gene. ATGL catalyzes the first reaction in lipolysis, in which triacylglycerols are hydrolyzed to diacylglycerols. ATGL has a very high substrate specificity for triacylglycerols and contains a catalytic dyad that utilizes a serine-aspartate nucleotide. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenate, cell culture supernatant |
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4.7 ★★★★★
Based on 2426 reviews
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Product Reviews
★★★★★ 5
Amazing
Amazing! Very easy to set up and use. Comes with pre heating options.
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Reviewed in the United States on May 18, 2025
★★★★★ 5
I Love the Typhur Sync wireless meat thermometer
Size: 2 Probes, Size: 2 Probes
I Love the Typhur Sync wireless meat thermometer. I bought it on sale and it is worth the money. It is exactly what I wanted and hoped for in a remote temperature sensor.
It also had a little paper that came with it with the factory tested calibrations listed for this unit.
I had a chance to do some initial testing with the Typhur Sync yesterday Feb 23rd 2024. I did a little runthough to get acquainted with it using a store bought already cooked Baby Back rack of ribs. Not the best choice as it was already cooked but at $10 from Costco it was ok to sacrifice. And it still turned out good.
I Cooked on a Weber Genesis E-330 propane grill and used a smoke tube. In the past I have used this propane gas grill to smoke Ribs, Port Shoulders and Brisket with a Smoke tube. It is possible using one side burner and a Smoke Tube.
I had also previously tried smoking with a custom smoker tray from Weber for my grill, which used wood chips, but that was mostly a failure. And I tried a Hot and Cold smoker unit on the back but it had limited success with the heat on.
But using a smoke tube and one burner on low you can do an ok smoke on the grill if you can verify your temperatures.
What I always struggled with was having faith in the grill hood's thermometer. It turns out the thermometer reads hotter than what the grill temps actually are around the meat! That resulted in an even slower low and slow cook and would not develope bark on pork shoulder.
Using the Typher Sync you can see the internal meat temps on 5 spots in the thermometer Probe plus it has one external ambient temperature sensor. So 6 total temps per Probe and you get two Probes!
When using the wifi mode it never looses signal. The base unit just should be kept close to the smoker, like 3 to 60 feet or so and it will relay the bluetooth data from the Probes immediately to the wifi so you can use the APP to see your status of the cook. Alternatively you can just use the base with wifi or bluetooth in a manual or smart mode.
When I did my test it did a firmware update prior to the start of the cook as it told me one was available to install. That took like 2 minutes since it was already in wifi mode. The firmware version I used was: 100016.
From the APP you can configure 1 or 2 Probes for individual use on the same hunk or meat or two separate items on the same or different grills with the same or different cooking parameters. It depends on how you set it up.
You choose the meat type and cut and the final temp to reach for that cut with some temperature recomendations that you can adjust.
It senses the internal temps from 5 internal sensors and displays an temps of all 5 and an average depending on if you are in Basic mode (white background with less details) or Professional Mode (black background and more details)
The Professional mode seems best for me. Seeing all the temps helps to verify that the Probe is placed in a good location. If it is improperly placed some readings may read too hot meaning it is too close to the surface or exposed. So in those cases, reposition the Probe. On a thick peice of meat the cent most sensor will read cooler than the others depending on their distance to the surface.
You can use the external sensors to help determin how to position the meet for your cooker or grill. All grills swwm to have hot and cooler areas. If it is cooler on one side or the other, reposition the meat to get it more equal. Or use the cooler side for the thinner end of the meat. This can also be verified by the checking internal temperatures or you can use both probes on the same piece of meat like I did for this rack of ribs which naturally has a thicher end and a thinner end.
I have included some of the pictures from the cook with some photos of the base unit and others in the App at various times on the cooking process.
The app displays a graph with the target internal ending temperature,
The current and past temperatures of each of the 5 interal sensors and the 1 external sensor.
Each Probe has its own graph line on the screen. You can slide back and forth between the Probes. You can also go back to the main status screen that shows basic stats of both Probes on one screen at the same time but no graphs or individual sensor readings. It main status screen shows the averaged current reading for internal tempurature, ambiant external temp, target temp and the estimated remaining time before reaching the target tempurature. This is the same as the base unit will display.
The Pro version of the app can be selected by toggling the icon in the upper righthand corner. It displays all the same information as the Basic mode and much more.
The Pro version displays separate graph lines for each of the 6 temperature sensors. It also displays an outline of the Prope near the top of the display along with the positions of all the sensors in the Probe!
You can press one of the temperature sensors locations and it will highlight its graph line. You can further slide your finger around that graph line and it displays the temperature at that position on the line!
But the App also warns you that your cook is within 5 minutes of completing with an autible sound if you have your notification sound volume turned up. It also displays a message on top of the current APP screen display. And when you start the cook it will warn that your notification sound is not turned on or up enough.
It has messages for each Probe for 5 minute before the cook ends, when the Cook completes, Please remove your food from heat, and Cooking complete after it is done with the 5 minute resting period. It also will remind you that your Probe was not returned to the base unit after a while of sitting in normal room temperatures!
Along the way those warnings alert you with a chime sound via your notification sound. It also warns you if your notification sound is turned off when you go to complete the setup of your cook. The base unit will also alert and sound for most of these steps as well. The sound on the base unit will stop when you acknowledge the warning/alert on the App or the unit.
The app and base unit also tracks the battery levels of the base unit and both Probes. During the 3 hours I played with it they were at Full the whole time.
The App also has a pretty extensive meat setting menu for selecting your type of meat: Beef Pork, Poultry, Lamb, Fish and Other and Manual setting. Other has Venison, Mutton, Kangaroo, Goat and Other! It also lets you select the type of cut for that meat with suggest temps for all cuts.
If appropriate it gives you temperature selections for Rare, Medium Rare, Medium, Medium Well, and Well done for most meats. And you can overide any of those to chose your own setting if you like it a bit more or less done.
The App also has recipes but there are not currently any taylored for use with a Typhur Sync.
But there is also an "Explore" section in the App that had some good articles. Make sure you press the arrow > link to see them all. It has topics like Reverse Sear Steak Temperature and Time,
Carryover Cooking: Remove Your Meat at a Lower Temperature than Target,
Best Tri Tip Temp: How to Know If Tri Tip is Done,
How Long to Grill Chicken Breast Perfectly Juicy
and various other guides.
The Typhur Sync is a very nice quality unit. It works well and it will be great for keeping track of a longer 10+ hour brisket or shoulder. Or even for shorter cooks in a air fryer or oven or grill where you want a particular internal temperature and do not want to over shoot it by waiting to long on a recheck with a hand held unit.
Please see all the photos for better detail. The place where the ambient temperature drops was from opening the grill hood to put some sauce on the ribs and check things.
Great Product!
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Reviewed in the United States on February 24, 2024
★★★★★ 5
Great Product, Even Better Customer Service
Size: 2 Probes, Size: 2 Probes
I purchased this product last August and had kept it in storage without using it even once. A few days ago, I finally took the thermometer out for its first use, but even after charging it, there was no response and it would not turn on at all. At first, I was very disappointed and confused because I had never even had a chance to use it.
I contacted customer service and provided a detailed report, including my purchase information, the condition of the device, and photos. Their team responded quickly and arranged to send me a replacement right away for the unit that had apparently become completely discharged and unusable. They shipped the new device the very next day and also provided the tracking number.
The replacement arrived safely a few days later, and I was very happy to use it today. The Typhur Sync WiFi Wireless Meat Thermometer is very convenient to use. I really like its clean design, app connectivity, and the way it provides accurate real-time temperature readings.
On top of that, Typhur’s customer service was clear, fast, and very helpful, which made my overall experience even more satisfying.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Great Wireless meat thermometer.
Size: 2 Probes
The Typhur Sync WiFi Wireless Meat Thermometer works great. One of my favorite features is that it’s completely wireless—it makes cooking so much easier without having to deal with tangled cords.
The phone app is a huge plus. It’s very convenient and versatile—you can use the preset settings or fully customize your cook. I especially like that you can set multiple alarms for different temperatures and situations. For example, I once forgot to check my wood chips and had an alert set if the temperature dropped below a certain point. Sure enough, the alarm went off, and when I checked, I was out of wood chips. That feature alone saved my cook.
I also find the app much easier to use than the actual unit when setting everything up. As for accuracy, the temperature readings between the two probes are within a degree of each other and match up with my instant-read thermometer, which gives me a lot of confidence in the results.
Overall, this is a reliable, user-friendly thermometer with great features. I’d definitely recommend it to anyone serious about grilling or smoking.
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Reviewed in the United States on April 28, 2026
★★★★★ 5
A Game-Changer for Perfect Grilling!
Size: 2 Probes
I've been using the Typhur Sync WiFi Wireless Meat Thermometer for a few months now, and it's hands down one of the best kitchen gadgets I've invested in. This thing is incredibly reliable, accurate, and convenient, making it a must-have for anyone who loves cooking meats to perfection without constant babysitting. Whether I'm smoking brisket low and slow or grilling steaks for a quick dinner, it delivers spot-on temperature readings every time, helping me avoid overcooking or undercooking disasters. I give it a solid 5 stars!
One of the cool features is that it comes with two probes, clearly labeled with numbers 1 and 2. Just a heads up—you have to start with probe 1 inserted first for the system to recognize and sync properly; it's a minor quirk but easy to remember once you get the hang of it. The probes themselves and base are battery-operated and include USB-C rechargeable batteries, which is super handy—no more hunting for disposable ones. The probes charge automatically when inserted into the base. Charging them is straightforward, and they hold up well during long cooks.
The real standout is the companion phone app, which elevates the whole experience. The wireless base unit connects to the probes via Bluetooth, but it can't be too far away from them, or they'll lose connectivity (I'd say keep it within 20-30 feet outdoors, depending on obstacles). That's where the app shines—it links to the base over WiFi, allowing you to monitor everything from your phone no matter how far a2ay you are, as long as the base is connected to the internet. I've checked on my roasts from the grocery store or even while running errands, getting real-time alerts for target temps or when it's time to rest the meat. It's freed me up to multitask without worrying.
One small downside is that the base takes a long time to charge fully—sometimes a few hours—and it does drain slowly even when not in use, probably due to some standby power draw. I've gotten into the habit of plugging it in regularly, even between uses, to keep it ready to go. It's not a deal-breaker, but worth noting for anyone who might forget.
Overall, for the price and performance, the Typhur Sync is worth every penny. It's made my BBQ sessions stress-free and consistently delicious. If you're serious about meat thermometry, grab this—you won't regret it! ⭐⭐⭐⭐⭐
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Reviewed in the United States on January 1, 2026