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Description
Human RAB37 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the Ras-related protein Rab-37 (RAB37). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Ras-related protein Rab-37 (RAB37) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ras-related protein Rab-37 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RAB37, also known as RAB37, is a member of the RAS oncogene family and is encoded by the RAB37 gene. Rab proteins are low-molecular-weight GTPases that are key regulators of vesicle trafficking. Reducing Rab37 levels by RNA interference results in impaired glucose-induced insulin secretion and a decrease in the number of granules tightly associated with the plasma membrane. It is a novel Rab GTPase specifically expressed in the MC-9 mast cell line and bone marrow mast cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.5 ★★★★★
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Product Reviews
★★★★★ 5
Lovely cabinet but needs time to put together!
Color: White, Size: 23x29
The overall cabinet is what I was looking for. Very sturdy and good looking. The main problem my husband had was with the directions to put it together. The pictures were hard to see and the majority of written directions were hard to understand. Definitely took longer to put together than should have taken. But when it was completed and hung, I have to say I love it. Be prepared to have patience and enough time set aside to put it together.
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Reviewed in the United States on January 10, 2026
★★★★★ 5
Solid cabinet; all aligns well
Color: White-1927, Size: 19x27
Amazon declined my first buy/ship if this cabinet as it was damaged when it reached Amazon. That was fine, but I still had to go thru ‘return’ process of Amazons, w/o a product to return; so I used their ‘chat feature’ to explain—went smoothly, and they secured the replacement for me; I just need to check latest bill to be sure I was only charged once. OK, now onto the cabinet: Solid materials (for MDF-which I generally avoid), and assembled + installed in just 1hr. Btw, I add ‘glue’ the the provided 8 wood dowels used for assembly. The doors hinge adjustments are easy to align doors for final finish. While this cabinet ‘hangs’ on its cleat, I did add one screw to the inside-bottom of cabinet to ensure no one inadvertently may ‘lift’ the cabinet off its cleat. This is rock solid secured to me wall above toilet for great extra storage in the kids’ bath! Overall, a good buy; but did expect it to be cheaper price considering MDF material and made in China. I look for country of origin now and try to buy from US friendly countries. Good luck, Mark in NY.
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Reviewed in the United States on April 1, 2024
★★★★★ 5
Very impressive
Color: Navy Blue, Size: 23x29
I've had a lot of experience with modern furniture that you have to put together. Most of it, I worry about the durability of the product. Most is put together with repurposed particle board. So it's heavy and I would think not durable - especially in a damp environment.
Saying that, I was very impressed with this product.
My experience with the product to this point has been the best of those type of products. It shipped very well...everything in place and neatly packed. The engineered wood (resembles plastic) seems solid, water resistant at a minimum, and scratch resistant. The instructions provided - while the product was easy to assemble - were amongst the clearest I've seen from such a product.
What impressed me the most was the thought behind the design of this product. The hinges are perfect for the product. The doors can be easily removed by pushing a button on the hinge. The holes on the boards were perfectly drilled - so much so that I didn't have to pound any board to put them together. The back wasn't nailed on and the slot used for the backing was spot on.
If I had to choose negatives (and I'm really nit-picking because this is really designed that well) it was that the shelf ends up being a bit loose and I would have liked the mounting board to match the width of the slot it was being used to fill. Both can be fixed at the house by home products and screws.
I was so impressed that I would go and buy another product from this manufacturer (and look for the manufacturer by name). IT is that good.
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Reviewed in the United States on December 4, 2022
★★★★★ 5
Great looking, nice quality
Color: Black, Size: 23x29
There is some assemble required. if your not ready to assemble this product may not be for you.
the machining was spot on, all parts went together as they should.
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Reviewed in the United States on November 24, 2025
★★★★★ 5
Stylish, sturdy cabinet
Color: White
This cabinet was even nicer than I thought it would be—stylish, sturdy, and easy to assemble. Perfect for holding medicines and small items in the bathroom, easy to see and find, out of reach of children.
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Reviewed in the United States on May 8, 2026
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